2.6 Multi-omics analysis of transcriptome, miRNAome, proteome, and metabolome
ACGT101-CORR 1.1 (LC Sciences, USA) was used to construct the negative miRNA-mRNA regulatory network (up-down and down-up). In brief, transcriptome and miRNAome were integrated by combining the expression profiles of DEG and DEMI with miRNA-targeting information. Afterwards the protein informations added miRNA-mRNA pairs to the miRNA-mRNA-protein pairs (up-down-down and down-up-up) directly. Secondly, 145 miRNA-mRNA-protein pairs and 33 DEM were combined to recognite mutual KEGG pathways. Finally, cytoscape software was mapped to generate an interaction network among the relationships of DEMI, DEG/DEP, DEM (Wenet al. 2019).