2.3 Differentially expressed miRNAs (DEMI) analysis
miRNA-seq method was the same as description in our previous article, including six small RNA libraries construction, illumina Hiseq 4000 sequencing, miRNA identification, prediction of target genes of miRNAs (Zhang et al. 2019). DEMI based on standardized deep-sequencing counts was analyzed by Student’s t-test (P < 0.05) (Li et al. 2017; Zhao et al. 2017).