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Proteome-wide identification of S-sulfenylated cysteines in Brassica napus
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  • Sidra Iqbal,
  • Liangqian Yu,
  • Yuting Zhang,
  • Guofang Zhang,
  • Usman Ali,
  • Shaoping Lu,
  • Xuan Yao,
  • Liang Guo
Sidra Iqbal
Huazhong Agricultural University

Corresponding Author:siqbal@uoswabi.edu.pk

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Liangqian Yu
Huazhong Agricultural University
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Yuting Zhang
Huazhong Agricultural University
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Guofang Zhang
Huazhong Agricultural University
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Usman Ali
Huazhong Agricultural University
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Shaoping Lu
Huazhong Agricultural University
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Xuan Yao
Huazhong Agriculture University
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Liang Guo
azhong Agricultural University
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Abstract

Deregulation of reduction-oxidation (redox) metabolism under environmental stresses results in enhanced production of intracellular reactive oxygen species (ROS), which ultimately leads to posttranslational modifications (PTMs) in structure and molecular function of responsive proteins. Redox PTMs are important mediators of cellular signalling and regulation and several proteomic approaches attempted to quantify them under various stresses in plants. We aimed to generate large-scale redox proteomics data in response to short-term salt stress in Brassica napus by analyzing reversible cysteine modification. We employed iodoTMT approach to analyze the redox proteome of Brassica napus seedlings under control and salt stress conditions. We identified 2,010 peptides in 1,017 proteins, of which 1,821 sites in 912 proteins had oxidative modification. The redox homeostasis of biology processes in chloroplast and cytoplasm were mainly affected and the modification levels of proteins involved in photosynthesis and glycolysis pathways were significantly changed. Two oxidatively modified proteins were selected from the candidates and their in vitro activity under oxidative stress was assayed and validated the findings of this proteomics study. This targeted approach should contribute to the understanding of redox-based molecular changes prevailing in Brassica napus proteome subjected to salt stress and the mechanism adopted to cope with it.