Mutant analysis
T-DNA lines for candidate genes were obtained from NASC and are listed
in Table S4. Genomic DNA was extracted using the Sucrose Prep method
(Berendzen et al., 2005), following genotyping by PCR for the respective
T-DNA insertion using the primer pairs listed in Table S8. Homozygous
mutant lines were tested on the same conditions used for the N screening
experiments at which the genome-wide association was identified, using
Col-0 wild type as a reference. Two and three independent experiments
with 10 replicate plants/line for each condition were performed to
confirm the associations to FT and ERD plasticities, respectively.