3.3. Enhancement of cell viability using 3D culture system
The viability of DPSCs grown in 2D or 3D scaffolds was assessed using the AB assay. After 24 h of cell culture (Fig. 3), there was a significant increase in the cellular activity of SA and GOSA scaffolds seeded with DPSCs compared to DPSCs grown under 2D conditions at all cell densities (\(p<0.0001\)). Cells seeded directly on the surface had an average (SEM) AB reduction of 53.7%±1.00 across all five seeding densities. Furthermore, cells on 3D scaffolds were found to be more viable when compared to the no scaffold (2D) condition (Fig. 3).