2.4. In vitro biodegradation study
The in vitro biodegradation study aims to investigate the biodegradation rate of prepared scaffolds [35]. Briefly, each scaffold (\(n=3\)) with known weight (\(W_{0}\)) was incubated at 37°C in alpha-MEM culture media containing 1% (v/v) penicillin/streptomycin to prevent bacterial growth. At specific time points, scaffolds were rinsed with ddH2O. Then, samples were dried under vacuum and weighed (\(W_{1}\)). The extent of biodegradation was calculated using the following formula.
In vitro Biodegradation (%)\(=\frac{W_{0}-W_{1}}{W_{0}}\times 100\)