Somatic Cell Source Reprogramming Factors Delivery Method Morphology Characteristics Final Products Specific Conditions Comments Year [Ref]
PBMC
Oct3/4, Klf4, Sox2, and c-Myc
Polycistronic lentiviral vector
A normal karyotype, stained positive for the pluripotency markers Oct4, Nanog, and Tra1-81. characteristic for all three germ layers.
iPSCs-derived T cells
Cells were expanded and reprogrammed more efficiently in the presence of IL-7 than cells grown in the presence of G-CSF, GM-CSF, IL-3, and IL-6.
TCR gene rearrangement test showed that none of the iPSCs were originated from B lymphocytes. The reason may associate with culture conditions used in this study, which primarily expanded the T lymphocytes and myeloid cells.
(Staerk et al., 2010)
PBMC Oct3/4, Klf4, Sox2, and c-Myc Temperature-sensitive mutated Sendai viral vector Positive for Nanog, Oct4, SSEA3, SSEA4, Tra-1-60, and Tra-1-81 Proteins. They were ALP-positive and displayed hESC-like colonies. iPSCs-derived T cells PBMCs were stimulated with a plate-bound anti-CD3 monoclonal antibody and recombinant IL-2. This study highlighted an easy way of iPSC generation from peripheral blood, specifically from myeloid cells. (Seki et al., 2010)
PBMC Oct3/4, Klf4, Sox2, and c-Myc Two lentiviruses, each encoding two reprogramming factors iPSC-derived PB colonies were positive for Tra-1-81, Nanog, Oct4, Tra-1-60, SSEA4, and ALP. Peripheral blood-derived iPSCs NA All of the generated iPSC clones from PBMC were positive for TCR rearrangement, signifying that none of the lines originated from B lymphocytes. (Loh et al., 2010)
Melanoma TILs Oct3/4, Klf4, Sox2, and c-Myc Sendai viral vector Positive for pluripotency and surface markers (SSEA3, SSEA4, Tra-1-81, Tra-1-60, and Oct4). And could differentiate in vitro and in vivo. TIL-derived iPSCs TILs were stimulated with IL-2 alone for 3-4 weeks; after starting expansion, they were stimulated with CD3/CD28 mAb. Heterogenous melanoma-specific TCR gene rearrangement was confirmed in iPSC-derived TIL, suggesting a new source of tumor-specific T cells for cancer patients. (Saito et al., 2016)
CD8 CTL Oct3/4, Klf4, Sox2, and c-Myc Sendai viral vector A normal karyotype, and showed pluripotency in the teratoma formation assay. iPSC-derived CD8αβ T cells Dexamethasone increases the expression of IL-7 receptor that contributes to anti-apoptotic survival and positive selection of CD8αβ T cells. CRISPR-mediated RAG2 knockout in iPSCs would preserve the antigen specificity of TCR in iPSC-derived T cells. This study also reported the differentiation of iPSCs into CD8αβ T cells. (Minagawa et al., 2018)