PBMC
|
Oct3/4, Klf4,
Sox2, and c-Myc
|
Polycistronic lentiviral vector
|
A normal karyotype, stained positive for the pluripotency markers Oct4,
Nanog, and Tra1-81. characteristic for all three germ layers.
|
iPSCs-derived T cells
|
Cells were expanded and reprogrammed more efficiently in the presence of
IL-7 than cells grown in the presence of G-CSF, GM-CSF, IL-3, and
IL-6.
|
TCR gene rearrangement test showed that none of the iPSCs were
originated from B lymphocytes. The reason may associate with culture
conditions used in this study, which primarily expanded the T
lymphocytes and myeloid cells.
|
(Staerk et al., 2010)
|
PBMC |
Oct3/4, Klf4, Sox2, and c-Myc |
Temperature-sensitive
mutated Sendai viral vector |
Positive for Nanog, Oct4, SSEA3, SSEA4,
Tra-1-60, and Tra-1-81 Proteins. They were ALP-positive and displayed
hESC-like colonies. |
iPSCs-derived T cells |
PBMCs were stimulated with
a plate-bound anti-CD3 monoclonal antibody and recombinant IL-2. |
This
study highlighted an easy way of iPSC generation from peripheral blood,
specifically from myeloid cells. |
(Seki et al., 2010) |
PBMC |
Oct3/4, Klf4, Sox2, and c-Myc |
Two lentiviruses, each
encoding two reprogramming factors |
iPSC-derived PB colonies were
positive for Tra-1-81, Nanog, Oct4, Tra-1-60, SSEA4, and ALP. |
Peripheral blood-derived iPSCs |
NA |
All of the generated iPSC clones
from PBMC were positive for TCR rearrangement, signifying that none of
the lines originated from B lymphocytes. |
(Loh et al.,
2010) |
Melanoma TILs |
Oct3/4, Klf4, Sox2, and c-Myc |
Sendai viral
vector |
Positive for pluripotency and surface markers (SSEA3, SSEA4,
Tra-1-81, Tra-1-60, and Oct4). And could differentiate in vitro
and in vivo.
|
TIL-derived iPSCs |
TILs were stimulated with IL-2
alone for 3-4 weeks; after starting expansion, they were stimulated with
CD3/CD28 mAb. |
Heterogenous melanoma-specific TCR gene rearrangement
was confirmed in iPSC-derived TIL, suggesting a new source of
tumor-specific T cells for cancer patients. |
(Saito et al.,
2016) |
CD8 CTL |
Oct3/4, Klf4, Sox2, and c-Myc |
Sendai viral vector |
A normal karyotype, and showed pluripotency in the teratoma formation
assay. |
iPSC-derived CD8αβ T cells |
Dexamethasone increases the
expression of IL-7 receptor that contributes to anti-apoptotic survival
and positive selection of CD8αβ T cells. |
CRISPR-mediated RAG2 knockout
in iPSCs would preserve the antigen specificity of TCR in iPSC-derived T
cells. This study also reported the differentiation of iPSCs into CD8αβ
T cells. |
(Minagawa et al., 2018) |