Figure 1. Schematic of experimental design, showing P. aegeria development through four larval instars to pupation. Larvae were divided between six treatments, consisting of exposure to short-day or long-day photoperiodic regimes at different points during larval development. Larvae were either exposed to constant daylengths (long control; short control), switched between daylength regimes (long to short; short to long) on the first day of the fourth larval instar, or switched between daylength regimes on the first day of the third larval instar.