2.2.3 Open Field (OF)
Emotional alterations and locomotor activity were evaluated by the OF
test using a white plywood apparatus (50 × 50 × 25 cm) as previously
described (Archer, 1973; Griñan-Ferré et al., 2016).The apparatus’
ground was divided into the center and peripheral area. Each individual
was placed at the centre of the open field and allowed to explore the
apparatus for 5 min. The apparatus was cleaned with 70% ethanol after
between trials. The behaviour was recorded and later analysed with SMART
ver. 3.0 software (Panlab). The locomotor activity of the mice
calculated as the sum of total distance travelled in 5 min, the centre
stay duration and the number of rearings were evaluated.