2.2.4 Elevated Plus Maze (EPM)
Animals were tested for anxiety-like behaviour by performing the EPM
test, based on a previously described protocol (Walf and Frye, 2007).
The EPM apparatus consisted of two open arms (30 x 5 x 15 cm) and two
closed arms (30 x 5 x 15 cm). The mice were placed at the junction of
the arms and allowed to explore the apparatus for 5 min freely. EPM
apparatus was cleaned with 70% ethanol between tests. The behaviour was
recorded and later analysed with SMART ver. 3.0 software (Panlab).
Parameters recorded included the total distance travelled during the 5
min test, the time spent in open arms, closed arms, and centre, as well
as the number of rearings.