2.8 Quantitative real‐time PCR
Total RNA was extracted from the liver tissues of the mice or LX-2 cells
using Tripure reagent (Roche Diagnostics, Indianapolis, IN) as described
by the manufacturer. cDNA synthesis was carried out with HiScript® II Q
Select RT SuperMix for qPCR (Vazyme). Quantitative PCR was performed in
biological triplicates using SYBR Green reagent (Vazyme). The level of
GAPDH (human) or β-actin (mouse) RNA expression was used to normalize
the data. PCR primer sequences are listed in Supplementary Table 1. A
melting curve of each amplicon was determined to verify its specificity.