2.8 Quantitative real‐time PCR
Total RNA was extracted from the liver tissues of the mice or LX-2 cells using Tripure reagent (Roche Diagnostics, Indianapolis, IN) as described by the manufacturer. cDNA synthesis was carried out with HiScript® II Q Select RT SuperMix for qPCR (Vazyme). Quantitative PCR was performed in biological triplicates using SYBR Green reagent (Vazyme). The level of GAPDH (human) or β-actin (mouse) RNA expression was used to normalize the data. PCR primer sequences are listed in Supplementary Table 1. A melting curve of each amplicon was determined to verify its specificity.