2.13 Statistical analysis.
The results are presented as mean ± SEM. Statistical differences between
two groups were analyzed by the unpaired Student’s t test with a
two-tailed distribution. Differences between multiple groups of data
were analyzed by one-way ANOVA with Bonferroni correction (Graph Pad
Prism 8.0, San Diego, CA, USA). P-values less than 0.05 were considered
statistically significant. Statistical analysis was undertaken only for
at least five independent experiments and other data was not
statistically analysed owing to group sizes of n < 5. The data
were analysed using one‐way ANOVA with Tukey’s post hoc test when F
achieved P < 0.05 and there was no significant variance in
homogeneity. The results were considered statistical significance at P
< 0.05. The data and statistical analysis comply with the
recommendations of the British Journal of Pharmacology on experimental
design and analysis in pharmacology(Curtis et al., 2018).