2.13 Statistical analysis.
The results are presented as mean ± SEM. Statistical differences between two groups were analyzed by the unpaired Student’s t test with a two-tailed distribution. Differences between multiple groups of data were analyzed by one-way ANOVA with Bonferroni correction (Graph Pad Prism 8.0, San Diego, CA, USA). P-values less than 0.05 were considered statistically significant. Statistical analysis was undertaken only for at least five independent experiments and other data was not statistically analysed owing to group sizes of n < 5. The data were analysed using one‐way ANOVA with Tukey’s post hoc test when F achieved P < 0.05 and there was no significant variance in homogeneity. The results were considered statistical significance at P < 0.05. The data and statistical analysis comply with the recommendations of the British Journal of Pharmacology on experimental design and analysis in pharmacology(Curtis et al., 2018).