2.7 Statistical analysis
All flow cytometry data were analyzed with FACS DIVA v8.0.1 (BD Biosciences) and FlowJo v10 software packages (FlowJo LLC, Ashland, OR, US). Statistical analyses were performed using Graphpad Prism (v8.4.1): the non-parametric Wilcoxon signed rank test for paired data and the non-parametric Mann-Whitney U-test for unpaired data. Receiver operating characteristic (ROC) curves were used to determine specificity and sensitivity of BAT or CytoBas for distinguishing between control and allergic patients. For all tests, p<0.05 was considered significant.
RESULTS