FIGURE
4. Sensitivity and specificity of BAT and CytoBas for allergy. A)Frequencies of CD63+ basophils following in
vitro stimulation with [Api m 1]4 (left) and
receiver operator characteristics (ROC) curve of CD63 positivity
(right). For each individual the highest frequency was selected from the
serial dilutions in Fig 2B . B) Frequencies of
CD63+ basophils following in vitro stimulation
with [Lol p 1]4 and ROC curve of CD63 positivity
(right). For each individual the highest frequency was selected from the
serial dilutions in Fig 3B . C) Staining intensities as
ratio of [Api m 1]4-APC/streptavidin-APC on
basophils (left) and ROC curve of [Api m 1]4staining intensity (right). D) Staining intensities as ratio of
[Lol p 1]4-APC/streptavidin-APC on basophils (left)
and ROC curve of [Lol p 1]4 staining intensity
(right). Statistics for CD63+ frequencies and allergen
tetramer stains, Mann-Whitney U-test; for ROC curves, Wilson/Brown
method to test whether the confidence level of the outcome distribution
is greater than 95%. **** p < 0.0001.