FIGURE
5. Multiplex flow cytometry using multiple allergen tetramers in a
single tube as a component-resolved diagnostic test for allergy.A, B) Representative histograms of basophils from a control
(blue) or RGP-allergic patient (red) stained with Lol p 1 and Lol p 5
tetramers in a single tube. C) 2D dot plot of Lol p 1 and Lol p
5 tetramer staining of the basophils from the control and patient in
panels A and B. D) Scatterplot depicting MFI ratios of
allergen:streptavidin for [Lol p 1]4-APC vs
strep-APC and [Lol p 5]4-BV711 vs strep-BV711 on
basophils from controls (blue, n = 10) and RGP-allergic subjects (red,
n=15) stained with both allergen tetramers or both streptavidin
conjugates in a single tube.