Aim of Study: Our aim is to compare dynamic thiol/disulphide homeostasis between patients with acute pulmonary thromboembolism (PTE) and healthy controls. Methods: Our study included 45 patients who were diagnosed with acute PTE and 50 healthy controls. Serum thiol/disulphide was measured. Results: We found that the native thiol, total thiol, native thiol/total thiol levels were significantly lower in the patient group than the control group, while the disulphide, disulphide/natural thiol, disulphide/total thiol levels were significantly higher. Conclusion: We have seen that in patients diagnosed with acute PTE, the oxidant-antioxidant balance shifts towards the oxidative direction. The disulfide/natural thiol ratio can be considered as an oxidative stress parameter in acute PTE. We think that the deterioration in thiol disulfide balance, together with clinical, laboratory and radiological findings, may have diagnostic value in acute PTE patients.