Objectives: The aim of this study was to analyze the frequency and results of congenital heart defects (CHD) in multipl pregnancies by fetal echocardiography. Method: This retrospective study was conducted at our hospital between January 2016 and September 2019. A total of 9440 pregnant women were enrolled in this study, including 241 were multiple pregnancies. (232 twins, 7 triplets and 2 quadruplets). Results: The prevalence of CHD was significantly higher in twin pregnancies (p=0.05). The rate of CHD was present in singletons 4.31% and twins 8.7%. There was no statistically significant difference between the two groups with respect to rate of critical congenital heart disease (p=0.32). Conclusion: Multiple pregnancies had increased congenital heart defect risk compared with singleton. Therefore, it is important to refer for fetal echocardiography when necessary.