Figure 5: Flow cytometric analyses of phagocytosis and efferocytosis. Unstained macrophage cells culture on control, M13phage, M13phage-RGD and gelatin surfaces for 2 and 7 days after co-cultured for 1 hours with stained yeast (PE-red) or stained apoptotic cells (PE-red) and fluorescence images of analyses of phagocytosis and efferocytosis. Unstained macrophage cells culture on control, M13phage, M13phage-RGD and gelatin surfaces for 2 and 7 days after co-cultured for 1 hours with stained yeast (DAPI-blue) or stained apoptotic cells (DAPI-blue).
(A) Flow cytometric and fluorescence images analyses of macrophages efferocytosis. Representative histogram shows the macrophage marker PE indicating efferocytosis.
(B) Flow cytometric fluorescence images analyses of macrophages phagocytosis. Representative histogram shows the macrophage marker PE indicating Phagocytosis.