Figure 3: iNOS and ARG1 expression, nitric oxide (NO) production in peritoneal macrophages from 4 groups experimental. (A) Amplicons densitometrically quantified; bars represent the relative amounts of amplified arginase-1 mRNA normalized against GAPDH, RT-PCR analysis for the expression of arginase-1 mRNA. (B) Amplicons were densitometrically quantified; bars represent relative amounts of amplified iNOS mRNA normalized against GAPDH. (C)Comparison of NO production, in macrophages at 2 and 7 days. (D) Comparison of levels of expression of iNOS with ARG1 mRNAs at 2 and 7 days. Graph shows collective data (*; Significant at P-value<0.05, **; Significant at P-value<0.01, ***; Significant at P-value<0.001). (n=3)