2.16 Data and statistical analysis
The data and statistical analysis comply with the recommendations of theBritish Journal of Pharmacology on experimental design and analysis in pharmacology (Curtis et al., 2018). All data in the text, tables, and figures are presented as means ± SD or means ± SEM. Statistical analysis was performed using GraphPad Prism 5 (GraphPad Software, Inc., La Jolla, CA, USA, RRID:SCR_002798). Statistical significance was determined by using two-way ANOVA or unpaired two-tailed Student’s t -test. P values < 0.05 were considered statistically significant. Representative data are presented as raw data. Unless indicated otherwise, five or more independent experiments were performed. Blinding or randomization was undertaken in this study.