FIGURE 5 TA inhibits the STAT3 signaling pathway in vivo . (a) Left: STAT3-regulated protein expression in tumor tissues. The protein levels of STAT3, p-STAT3 (Tyr705), Survivin, Bcl-2, c-Myc, ICAM-1, and Cyclin D1 in the tumor tissues were assessed using western blotting. Right: Analysis of the results in (a). Protein levels were quantified by using ImageJ software. Data are shown as means ± SD (n = 5). *P < 0.05. (b) Left: Immunohistochemical analysis of tumor tissues. Tumor tissues were fixed, dehydrated, and embedded into paraffin blocks. Tissue sections were cut from paraffin blocks and immunolabeled to detect the expression of p-STAT3 (Tyr705), c-Myc, Survivin, MMP-9, and ICAM-1. Scale bar, 50 μm. Right: Analysis of the results in (b). Positive cells were counted by using ImageJ software. Data are shown as means ± SD (n = 5). *P < 0.05.