FIGURE 2 TA significantly inhibits the proliferation and growth of pancreatic cancer cells. (a) Chemical structures of Trienomycins A, B, C, D, E, F, G, J, K, and L. (b) Effect of trienomycins on the proliferation of pancreatic cancer cells. Cells were treated with different concentrations of trienomycins (0-10 μM) for 48 h. Cell viability was measured with MTS assay. Data are shown as means ± SD (n = 5). (c) Effect of TA on the proliferation of pancreatic cancer cells. Cells were treated with different concentrations of TA (0-10 μM) for 48 h or 72 h. Cell viability was measured with MTS assay. Data are shown as means ± SD (n = 5). (d) Up: Effect of TA on the colony formation of PANC-1 cells. Cells were seeded into six-well plates and treated with different concentrations of TA (0-0.1 μM) for 7 days. Colonies were stained with crystal violet and photographed by using a digital camera. Down: Analysis of the results in (d). Colonies were counted by using ImageJ software. Data are shown as means ± SD (n = 5). *P< 0.05.