FIGURE 4 TA substantially suppresses pancreatic tumor growthin vivo . Male BALB/c nude mice were subcutaneously injected with PANC-1 cells and intraperitoneally administered with 10, 20, and 40 mg/kg/d of TA or vehicle control for 15 days. Gemcitabine hydrochloride (80 mg/kg/3d) was used as a positive drug. (a) Effect of TA on the pancreatic tumor growth. At the end of the experiment, the tumors were harvested. The picture was taken using a digital camera. (b) Tumor volume was measured once every 3 days. Data are expressed as means ± SD (n = 5). *P < 0.05. (c) Tumor weight was determined at the last day indicated. Data are expressed as means ± SD (n = 5). *P < 0.05. (d) Up: H&E staining and immunohistochemical analysis of tumor tissues. Tumor tissues were fixed, dehydrated, and embedded into paraffin blocks. Tissue sections were cut from paraffin blocks, stained with hematoxylin and eosin (H&E), or immunolabeled to detect the expression of Ki67 and PCNA. Scale bar, 50 μm. Down: Analysis of the results in (d). Positive cells were counted by using ImageJ software. Data are expressed as means ± SD (n = 5). *P < 0.05. (e) Body weight was measured once every 3 days. Data are expressed as means ± SD (n = 5). (f) Heart, liver, spleen, lung and kidney of mice were fixed, dehydrated, and embedded into paraffin blocks. Tissue sections were cut from paraffin blocks and used for H&E staining. Scale bar, 100 μm.