2.2. Viral clearance studies
Viral clearance studies on various components of the flow-through two column chromatography (AEX and CEX) and virus removal filter were conducted at ViruSure (Vienna, Austria) using purified mAb produced from the same cell line but by fed-batch production and purification with Protein A and IEX columns. The mAb used in these studies was 10 mg/mL mAb in 20 mM Tris-acetate, 10 mS/cm, pH 6.5. MVM and X-MuLV virus titers were determined by TCID50 method.