2.2. Viral clearance studies
Viral clearance studies on various components of the flow-through two
column chromatography (AEX and CEX) and virus removal filter were
conducted at ViruSure (Vienna, Austria) using purified mAb produced from
the same cell line but by fed-batch production and purification with
Protein A and IEX columns. The
mAb used in these studies was 10 mg/mL mAb in 20 mM Tris-acetate, 10
mS/cm, pH 6.5. MVM and X-MuLV virus titers were determined by
TCID50 method.