Beasley Lake Watershed is an agriculturally influenced drainage basin in western Mississippi that has been intensively studied for 25 years. As part of the USDA Conservation Effects Assessment Project (CEAP), the watershed has archived hydrology, precipitation, and water quality data in order to measure the effects of multiple USDA Natural Resources Conservation Service conservation practices on lake water quality. The long-term database is available to researchers using a web-based application, Sustaining the Earth’s Watersheds, Agricultural Research Data System (STEWARDS). STEWARDS is a GIS-based data retrieval application that encompasses spatial and temporal data collected from multiple sites within the watershed. This data note describes information located in the STEWARDS Beasley Lake Watershed database, including hydrology, precipitation, and water quality data. This information is valuable to researchers and agencies beyond the USDA as an available and useful database to improve the understanding of how land-use practices affect the water quality of shallow lake systems.