Infertility secondary to chemotherapy and/or myeloablative conditioning regimens prior to stem cell transplantation is an important cause of morbidity and psychosocial distress among pediatric cancer patients. Known options exist for fertility preservation; however, knowledge among providers varies. We conducted a pilot study with an educational intervention over one-hour for hematology-oncology faculty, fellows, and advanced practice providers. Participants completed pre-/post-test assessment on fertility preservation knowledge. Participants’ pre-test mean (SD) score was 53% (17%), which significantly increased to 72% (11%) in the post-test (P=0.0004). We demonstrated that a fertility education intervention could improve knowledge regarding infertility risk assessment and fertility preservation options.