Mathematical model
In order to simulate the multi-enzyme cascade reaction sequence in the
miniplant, a customized mathematical model is developed and solved in
Aspen Custom Modeler® V8.8 (Aspen Technology, Inc.)
using an equation-oriented approach for simulating. As shown in figure
3, this model is divided into three sub-models: aqueous phase, phase
interface, and organic phase. These sub-models are connected through
differential mass balance equations for each component. The reaction
kinetics used for the mathematical modeling of each enzyme have been
obtained through kinetic measurements.