Mathematical model
In order to simulate the multi-enzyme cascade reaction sequence in the miniplant, a customized mathematical model is developed and solved in Aspen Custom Modeler® V8.8 (Aspen Technology, Inc.) using an equation-oriented approach for simulating. As shown in figure 3, this model is divided into three sub-models: aqueous phase, phase interface, and organic phase. These sub-models are connected through differential mass balance equations for each component. The reaction kinetics used for the mathematical modeling of each enzyme have been obtained through kinetic measurements.