2.14 | Measurement of plasma cholesterol and triglycerides
Mice were sacrificed and blood was drawn from the right atrium into EDTA-containing tubes, for lipid measurements. Plasma was prepared via centrifugation at 850 ×g for 15 min at 4 °C and stored at -20 °C. Measurements were carried out using the Infinity Cholesterol and Triglycerides measurement kits (Thermo Scientific, USA, Cat# TR13421, Cat# TR22421), based on the absorbance of samples normalized to the absorbance of a known concentration of a calibrator provided with the kit.