Background: How to shorten the anesthesia time is an important problem to ERAS. The authors formulated a series of optimization measures and verified that these measures could shorten the anesthesia time and improve the operating room utilization efficiency by multidisciplinary diagnosis and treatment pattern. Methods: 90 patients were random selected to analyses the time of every preparing procedure and between 2 adjacent procedures, the multidisciplinary team established the optimization measures accordingly. Then 95 patients for gastrointestinal surgery received collaborative optimization measures that were established after discussion among anesthesiologists, surgeons and operating room nurses (optimization group), while 226 cases received the conventional approaches (control group). The data obtained from operating room monitoring recording of the two groups of patients were analyzed. Results: The preparation times from the initial of induction of anesthesia to start of surgery in optimization group were all significantly shorter than those in control group (all P<0.0001). In supine position surgery, the times from initial of induction of anesthesia to start of urinary catheterization, from urinary catheterization finish to start of disinfection, and from completion of draping to start of surgery in patients receiving optimization measures were all significantly superior to those receiving conventional approaches (all P<0.0001); in lithotomy position surgery, the times from the initial of induction of anesthesia to positioning, from positioning finish to start of disinfection, and from completion of draping to start of surgery in patients receiving optimization measures were all significantly superior to those receiving conventional approaches (all P<0.0001). Conclusions: The established optimization measures based on multidisciplinary team collaboration could significantly shorten the anesthesia time. The optimized measures in this study are feasible and effective to shorten the anesthesia time.