Calibration and testing for the cell counting approach
To establish the calibration relationship between biomass concentration
and cell counting results, we first conducted cell counting experiments
for each single culture (M. alcaliphilum 20ZR, S. sp.PCC7002, M. capsulatus and C. sorokiniana ). For each
strain, cell counting was performed for 4 different biomass
concentrations, with triplicates for each sample. To validate the
effectiveness of the cell counting approach, we prepared static
coculture samples by mixing given amounts of each individual
microorganisms together. For each coculture pair, six coculture samples
with different compositions were tested with triplicates.