We describe the first case of cryoballoon (CB) pulmonary vein isolation (PVI) facilitated by perioperative electroanatomical mapping (EAM) in a patient with dextrocardia due to Kartagener’s syndrome and highly symptomatic paroxysmal atrial fibrillation. Current case report highlights the different advantages of the addition of EAM to CB ablation for AF. This approach is feasible strategy for rhythm control in patients with atrial fibrillation and Kartagener’s syndrome for which medication can be contra-indicated.