Results of the preparation of biodegradable porous scaffolds using an original modification of a wet phase inversion method were presented. Influence of gelatin nano-non-woven as a non-classic pore precursor and polyvinylpyrrolidone, Pluronic® as classic pore precursors on the structure of obtained scaffolds was analyzed. It was shown that the addition of gelatin nano-non-wovens enables the preparation of scaffolds, which allow for the growth of cells (size, distribution, and shape of pores). Mechanical properties of the obtained cell scaffolds were determined. The influence of pore precursors on mass absorption of scaffolds against isopropanol and plasma was investigated. Interaction of scaffolds with a T-lymphocyte line (Jurkat) and with fibroblasts (L929) was investigated. Obtained scaffolds are not cytotoxic and can be used as implants, e.g. the regeneration of cartilage tissue.