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The ACID PHOSPHATASE 1 regulates Pi stress adaptation by maintaining intracellular Pi homeostasis
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  • Su Deng,
  • Jingyi Li,
  • Zezhen Du,
  • Zixuan Wu,
  • Jian Yang,
  • Hongmei Cai,
  • Gaobing Wu,
  • Fangsen XU,
  • Yichao Huang,
  • Sheliang Wang,
  • Chuang Wang
Su Deng
Huazhong Agricultural University

Corresponding Author:dengsuren@webmail.hzau.edu.cn

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Jingyi Li
Huazhong Agricultural University
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Zezhen Du
Huazhong Agricultural University
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Zixuan Wu
Huazhong Agricultural University
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Jian Yang
Sichuan University
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Hongmei Cai
Huazhong Agricultural University
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Gaobing Wu
Huazhong Agricultural University
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Fangsen XU
Huazhong Agricultural University
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Yichao Huang
Anhui Medical University
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Sheliang Wang
Huazhong Agricultural University
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Chuang Wang
Huazhong Agricultural University
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Abstract

The concentration and homeostasis of intracellular phosphate (Pi) are crucial for sustaining cell metabolism and growth. During short-term Pi starvation, intracellular Pi is maintained relatively constant at the expense of vacuolar Pi. After the vacuolar stored Pi is exhausted, the plant cells induce the synthesis of intracellular acid phosphatase (APase) to recycle Pi from expendable organic phosphate (Po). In this study, the expression, enzymatic activity and subcellular localization of ACID PHOSPHATASE 1 (OsACP1) were determined. OsACP1 expression is specifically induced in almost all cell types of leaves and roots under Pi stress conditions. OsACP1 encodes an acid phosphatase with broad Po substrates and localizes in the endoplasmic reticulum (ER) and Golgi apparatus (GA). Phylogenic analysis demonstrates that OsACP1 has a similar structure with human acid phosphatase PHOSPHO1. Overexpression or mutation of OsACP1 affected Po degradation and utilization, which further influenced plant growth and productivity under both Pi-sufficient and Pi-deficient conditions. Moreover, overexpression of OsACP1 significantly affected intracellular Pi homeostasis and Pi starvation signalling. We concluded that OsACP1 is an active acid phosphatase that regulates rice growth under Pi stress conditions by recycling Pi from Po in the ER and GA.
23 Aug 2021Submitted to Plant, Cell & Environment
23 Aug 2021Submission Checks Completed
23 Aug 2021Assigned to Editor
24 Aug 2021Reviewer(s) Assigned
03 Sep 2021Review(s) Completed, Editorial Evaluation Pending
06 Sep 2021Editorial Decision: Revise Minor
10 Sep 20211st Revision Received
13 Sep 2021Submission Checks Completed
13 Sep 2021Assigned to Editor
13 Sep 2021Review(s) Completed, Editorial Evaluation Pending
13 Sep 2021Editorial Decision: Accept