Binding of the fusion protein to GEM particles
Sf9 cells were inoculated with recombinant baculovirus at an MOI of 0.5,
and then harvested 4 days later. The supernatant was obtained by
centrifugation (1650 g/min for 30 min), and the fusion protein in the
supernatant was mixed with GEM on a rotary shaker at RT for 1 h. After
centrifugation at 6000 g/min for 10 min, the precipitate was washed 5
times with PBS and then resuspended in PBS to obtain fusion-GEM
complexes.