Immunofluorescence analysis (IFA)
For detection of the ZIKV-E protein, Sf9 cells were seeded in 24-well
plates and infected with recombinant baculovirus at an MOI of 0.5.
Forty-eight hours later, the cells were fixed with 70% cold ethanol and
stained with an anti-flavivirus E mAb (1:200) as the primary antibody.
Then, a FITC-conjugated anti-mouse antibody (1:200) and
Evans blue (1:300) were used. Fluorescence was observed under a
fluorescence microscope (Olympus Corp, Tokyo, Japan).