Table and Figure captions
TABLE 1 The relative abundance (%) of predominant genera in CV
and GF samples
FIGURE 1 The intestine length and weight of the faster
and slower regenerating Apostichopus japonicus individuals during
the different regeneration stages. The
differences
in
the intestine length averages of the two groups, ”faster” and ”slower”,
were statistically significant. ** indicates significant difference in
the intestine length averages of the two groups atP <0.01 level. Different letters indicate significant
differences (P <0.05) in the intestine weight.
Note: W-F: intestine weight of the faster regenerating A.
japonicus individuals; W-S: intestine weight of the slower regeneratingA. japonicus individuals; L-F: intestine length of the faster
regenerating A. japonicus individuals; L-S: intestine length of
the slower regenerating A. japonicus individuals.
FIGURE 2 The intestinal microbiota of the faster and slower
regenerating Apostichopus japonicus individuals are different.
(a) Comparison of the intestinal bacterial communities in the faster and
slower regenerating Apostichopus japonicus individuals during
different regeneration stages. (b) Differences in bacterial communities
at the family level between the faster and slower regenerating
individuals
(Wilcoxon
rank-sum test, Storey’s methods for multiple tests adjustment).
Note: F: faster regenerating group; S: slower regenerating group.
FIGURE 3 Heatmap of the KEGG analysis of the intestinal
microbiota in samples from the faster and slower regeneratingApostichopus japonicus individuals during the different
regeneration stages.
FIGURE 4 Quantification of 16S rRNA gene copies/sea
cucumber in the faster and slower regenerating Apostichopus
japonicus individuals during the different regeneration stages. qPCR
was performed with bacterial-specific primers on DNA extracted from the
F or S groups. Different letters indicate significant differences
(P <0.05).
FIGURE 5 Quantification of 16S rRNA gene copies/sea
cucumber during the different regeneration stages. qPCR was performed
with bacterial-specific primers on DNA extracted from the CV, XGF, or GF
groups. Different letters indicate significant differences
(P <0.05).
FIGURE 6 The intestine length (a) and weight (b) of
samples from the CV, XGF, or GF groups during the different regeneration
stages. (c) Images of regenerating intestines from the CV, XGF, or GF
groups at 10 or 14 d.
Different
letters indicate significant differences (P <0.05).
Note: CV1-XGF1-GF1: 10 d; CV2-XGF2-GF2: 14 d.