2.2 in vivo hair growth test
Six-week-old C57BL/6N male mice were purchased from Koatech Co. (Gyeonggido, Korea) and acclimated for 1 week. The hairs on the backs of 7-week-old mice, whose hair follicles were in telogen phase, were shaved with a hair clipper. For each of the mice, 300 μl of each drug was applied topically daily at an appropriate concentration (as described in figure legends) for up to 14 or 28 days. KY19382 was dissolved in a PEG400 and β-cyclodextrin (vehicle 1), and minoxidil was dissolved in 50% (vol/vol) ethanol, 30% water, and 20% propylene glycol (vehicle 2).