2.1 Cell culture and reagents
Primary human DP cells from passages 2-7 were used in this study (Kwack
et al., 2013). The cells were cultured in Dulbecco’s low glucose
modified Eagle’s medium (Hyclone, Pittsburgh, PA) supplemented with 10%
fetal bovine serum (Gibco, Gaithersburg, MD), 1% antibiotic-antimycotic
(Gibco), 1 ng/ml bFGF (Peprotech, Princeton, NJ), and 5 μg/ml insulin
(Gibco) at 37℃ in a humidified atmosphere of 5% CO2. A
rat vibrissa immortalized DP cell line was donated by the Skin Research
Institute of the Amore Pacific Corporation R&D Center (Yongin, Korea).
HEK293T cells containing a chromosomally integrated TCF reporter
(TOPflash) were cultured in Dulbecco’s Modified Eagle Medium (DMEM)
(Gibco) containing 10% (v/v) fetal bovine serum (Gibco) and 100 U/ml
penicillin/streptomycin (Gibco) at 37oC in a
humidified atmosphere of 5% CO2.