Pharmacokinetic analysis
Noncompartmental analysis was performed to calculate the PK parameters
of serum and tear EGF using Phoenix®WinNonlin® software version 8.0 (Certara, St. Louis,
MO, USA). The observed serum concentrations and times were used to
estimate the maximum concentrations (Cmax) and the
observed serum concentration after 12 hours of the administration
(C12h) of serum and tear EGF and the time to reach
Cmax (Tmax). The area under the
concentration-time curve from the pre-dose to 12 hours
(AUC0-12h) was calculated using the linear up/log down
trapezoidal method.