not-yet-known not-yet-known not-yet-known unknown Obesity has reached critical levels worldwide, driving comorbidities like type 2 diabetes, hypertension, dyslipidemia, and cardiovascular diseases. These conditions stem from adiposopathy, a pathological alteration of adipose tissue caused by hypercaloric diets and sedentary lifestyles. Current treatments, including pharmacotherapy and bariatric surgery, are limited by side effects, invasiveness, and poor patient compliance, highlighting the urgent need for alternative therapies targeting adiposopathy. In this study, we explore a novel magneto-mechanical treatment (MMT) using functionalized magnetic nanoparticles (MNPs) to induce mechanical stress in adipose tissue cells via a low-frequency alternating magnetic field (LF-AMF). We synthesized octahedral Fe3O4 MNPs through thermal decomposition and functionalized them with meso-2,3-dimercaptosuccinic acid (DMSA) and chitosan (CHITO) to enhance biocompatibility. The effects of MMT were evaluated on macrophages and adipocytes—key players in adiposopathy—using a custom-built LF-AMF device operating below 100 Hz. We demonstrate that MMT induces cellular damage in macrophages, reactive oxygen species (ROS) production, and inhibited cell proliferation and inflammation. In adipocytes, MMT promotes lipolysis, reducing fat storage and restoring physiological differentiation. These results suggest that MMT can modulate adipose tissue composition and function, offering a promising non-invasive approach to treating adiposopathy. Further studies are needed to elucidate the molecular mechanisms and optimize therapeutic application.

In this work, for the first time, snail slime from garden snails “Helix Aspersa Müller”, has been used to induce the formation of eco-friendly gold nanoparticles (AuNPs-SS), suitable for biomedical applications. An AuNPs-SS comprehensive investigation was performed observing AuNPs with an average particle size of 14±6 nm, stabilized by a slime snail-based organic layer. Indeed, as recognised in high-resolution MALDI-MS analyses, and corroborated by FESEM, UV-Vis, ATR-FTIR and XPS results, it was possible to assess the main presence of peptides and amino acids as main components of the slime, that, combined with the AuNPs confers them interesting properties. More specifically, we tested, in vitro, the AuNPs-SS safety in human keratinocytes and their potential effect on wound healing as well as their anti-inflammatory properties in Murine Macrophages. Moreover, the AuNPs-SS treatment resulted in a significant increase of the urokinase-type plasminogen activator receptor (uPAR), essential for keratinocyte adhesion, spreading and migration, together with the reduction of LPS-induced IL1-β and IL-6 cytokine levels, and completely abrogated the synthesis of the inducible nitric oxide synthase (iNOS).