2.5. Nuclear Magnetic Resonance Spectroscopy (NMR)
Analyses were performed on a Bruker Avance 500 MHz spectrometer. Spectra
were acquired at 300K, observing 1H at 500.128 MHz and13C at 125.75 MHz. Data were processed with Topspin
software package version 1.3. The samples were dissolved in
CDCl3. Spectra were acquired using 16 scans, 32 K data
points, 90 pulse angles (10.5 \(\mu\)s), relaxation delay 1 s,
acquisition time 2.47 s, and spectral width 13.2 ppm (6613.7 Hz).
Spectra were accurately phased, and their baseline adjusted accordingly.
Tetramethyl silane (TMS) was used as internal standard, and chemical
shifts were reported in ppm.