Figure 3. Consensus logo of top 50 abundant sequences from each selection round of mRNA display. A round-by-round consensus logo was created using Weblogo3 (Crooks et al., 2004) using the top 50 most abundant sequences based on their RPM. Sequence logos derived from FLAG-random library (A), 10aa-random library (B), core FLAG motif bearing sequences within the 10aa-random library (C and D) are color coded based on their charge (negatively charged: red, positively charged: blue and others: black). For the 4th round, consensus logo was only created for the sequences eluted with highest FLAG epitope tag concentration (100 μg/ml).