Fig. 1: (a) Multiple sequence alignment of PEBP-like
protein sequences. Sequences were aligned using the MUSCLE alignment
tool. The red asterisks indicate the conserved and key amino acid
residues required for the corresponding activity of FT and TFL1-like
sequences. The band at the bottom of the alignment represents the
percentage of conservation of the amino acids at a position. Red
indicates stronger conservation while blue represents variable
sequences. (b) Phylogenetic reconstruction of the
PEBP-gene family with homologous C. pallens sequences . The
evolutionary history was inferred by using the Maximum Likelihood method
based on the JTT matrix-based model. The bootstrap consensus tree
inferred from 1000 replicates is taken to represent the evolutionary
history of the taxa analysed. Aly: Arabidopsis lyrata subsp.
lyrata, Ath: Arabidopsis thaliana , Ac: Allium cepa, Bv:Beta vulgaris, Bd: Brachypodium distachyon, Cs:Chrysanthemum seticuspe , Chm: Chrysanthemum X morifolium,Gm: Glycine max, Ha: Helianthus annuus, Nt:Nicotiana tabacum , Osa: Oryza sativa, Ptr:Populus trichocarpa, ZCN: Zea mays and C. pallens .
Branches in red colour represent the MFT clade, blue represent theTFL1
clade and green represent the FT clade. (c) Functional
characterisation of the C. pallens PEBP- like sequences. The
number of rosette leaves of the transgenic and the wild‐type plants at
flowering (mean ± SD, n = 10). (Significance of transgenic and control
plants compared to Ler ft-1 , Student’s t-test *** P <
0.001, ** P <0.01).