loading page

Permutation of M2e affects the effectiveness of universal influenza nanovaccine
  • +6
  • Peiyang Ding,
  • Gaiping Zhang,
  • Yumei Chen,
  • Hongliang Liu,
  • Yunchao Liu,
  • Rui Jia,
  • Yanwei Wang,
  • Ge Li,
  • Aiping Wang
Peiyang Ding

Corresponding Author:dingpeiyang1990@163.com

Author Profile
Gaiping Zhang
Zhengzhou University
Author Profile
Yumei Chen
Author Profile
Hongliang Liu
Author Profile
Yunchao Liu
Henan Acad Agr Sci
Author Profile
Rui Jia
Zhengzhou University
Author Profile
Yanwei Wang
Author Profile
Aiping Wang
Zhengzhou University
Author Profile

Abstract

Influenza A virus (IAV), a deadly zoonotic pathogen, occasionally cross-species transmission among humans, swine and avian. The ectodomain of matrix protein 2 (M2e) is highly conserved in IAV and has been widely concerned in the development of universal influenza vaccines. Due to low immunogenicity, multi-copy M2e are usually displayed on the surface of nanoparticles to constitute universal nanovaccines. Here, we report that the permutation of the M2e affects the immune effect of the nanovaccine. Three M2e derived from humans, swine and avian IAV were inserted into the C-terminal of the Cap protein of porcine circovirus type 2 (PCV2) to form self-assembled nanovaccine. Immunoprotective effects of different M2e arrangements were explored in mice. Results showed that the M2e closest to the surface of nanoparticle induced the most efficient protection against IAV derived from corresponding species. The results will help in the development of more effective universal influenza vaccines, especially for specific species.