Antibody measurements
Serology tests for HBsAg, HBsAb, hepatitis B e antigen, hepatitis B e
antibody, and hepatitis B core antibody were performed quantitatively
using ELISA in all children before transplantation and
at
nearly 1 month, 3 months, 6 months and 12 months after transplantation.
Blood samples were also collected to evaluate HBV serology status in
donors before transplantation. HBsAb titers were defined as negative at
levels <10 IU/L and positive at ≥10 IU/L; positive titers were
considered to confer protective
immunity against HBV.