Selection and mating of female tsetse for the experiment
The G. m. morsitans colony at LSTM is maintained in 12 trays corresponding to each week of life. Females in the colony older than this are killed due to a decline in reproductive output. To select females for the experiment, we collected equal numbers of pupae from each tray over a one-week period for emergence (S2 File). At the time of mating (upon emergence or after a delay), females were mated at a ratio of 2:1 (female:male) with one-week old males for 48 hours. Males were then removed, and females were selected for the experimental treatments.