Macroscopic and Histopathological Examination in Animal Studies
With increasing the dosage of ethanol (0.2, 0.3, 0.5ml), the lesion size also increased (Figure 6A, Supplementary Table 1). Histology examination revealed both acute and chronic homogenized necrosis of the myocardium. Acute histology examination demonstrated a cloudy swelling of muscle with the disappearance of some of the nuclei, and the cross-striation of myofibrils was decreased (Figure 6B). The chronic lesion showed homogeneous fibrosis with clear but irregular border (Figure 6C).