Ethanol Injection in Animal Experiments to study acute and
delayed effect
Intramyocardial ethanol injection was tested in four dogs in order to
figure out the morphology and histology of the acute lesions and to
verify the relationship between the injection dosage and the lesion
size. After general anesthesia, the heart was exposed by left
thoracotomy. Boluses of ethanol were injected at LV anterior wall at a
dose of 0.2 ml, 0.3 ml and 0.5 ml for each dog. Two dogs were sacrificed
after 15 minutes after finishing the injections to study the acute
effect. The other two dogs would be sacrificed after 6 weeks to study
the delayed effect. Morphologic and histopathological examinations were
performed for each specimen. The animal study was approved by the animal
ethic committee of Nanjing Medical University.