Macroscopic and Histopathological Examination in Animal Studies
With increasing the dosage of ethanol (0.2, 0.3, 0.5ml), the lesion size
also increased (Figure 6A, Supplementary Table 1). Histology examination
revealed both acute and chronic homogenized necrosis of the myocardium.
Acute histology examination demonstrated a cloudy swelling of muscle
with the disappearance of some of the nuclei, and the cross-striation of
myofibrils was decreased (Figure 6B). The chronic lesion showed
homogeneous fibrosis with clear but irregular border (Figure
6C).