Ethanol Injection in Animal Experiments to study acute and delayed effect
Intramyocardial ethanol injection was tested in four dogs in order to figure out the morphology and histology of the acute lesions and to verify the relationship between the injection dosage and the lesion size. After general anesthesia, the heart was exposed by left thoracotomy. Boluses of ethanol were injected at LV anterior wall at a dose of 0.2 ml, 0.3 ml and 0.5 ml for each dog. Two dogs were sacrificed after 15 minutes after finishing the injections to study the acute effect. The other two dogs would be sacrificed after 6 weeks to study the delayed effect. Morphologic and histopathological examinations were performed for each specimen. The animal study was approved by the animal ethic committee of Nanjing Medical University.