2.7.2 Open-field test
Spontaneous exploration and locomotor activity were assessed in the open field test (Seibenhener & Wooten, 2015). A Plexiglas arena (50 x 50 cm and 38 cm high) was equipped with a video tracking system (SMART 3, Panlab Harvard Apparatus, Spain) to monitor animal activity. The arena was wiped with 75% ethanol before and after each test to eliminate scents left by other mice. Mice were placed individually in the center of the apparatus at the beginning of each test and allowed to explore freely for 10 min. Total distance traveled, mean travel speed, and number of center zone entries were determined by the software.