Sample preparation
For bone marrow sampling, after obtaining written informed consent from
two donors and reviewing histories including history of malignant
diseases, autoimmune diseases, hypertension, diabetes and history of
drug use, traveling abroad and other important aspects, human
Immunidificiency virus 1,2, Hepatitis B virus evaluation, Hepatitis C
virus evaluation, diagnostic tests for evaluation of Treponema Pallidum
(syphilis), Toxoplasma Gond and Cytomegalovisus were performed for
patients. After examining the results of the test and excluding all risk
cases, 2 to 5 ml bone marrow aspiration were prepared and were
transferred to the laboratory under sterile conditions in a
heparin-containing tube.