Determination of TNFR, DNAM- and ST2 levels by ELISA
First, all reagents and samples reached room temperature before use. TNFR, DNAM-1 and ST2 levels were measured according to the protocols of Quantikin ELISA respectively. From all patients’ samples, controls and standards two samples were prepared. Tests were carried out according to the protocol. Based on OD results the mean results was counted. Log/lag curve was used for showing the results.