Sample preparation
For bone marrow sampling, after obtaining written informed consent from two donors and reviewing histories including history of malignant diseases, autoimmune diseases, hypertension, diabetes and history of drug use, traveling abroad and other important aspects, human Immunidificiency virus 1,2, Hepatitis B virus evaluation, Hepatitis C virus evaluation, diagnostic tests for evaluation of Treponema Pallidum (syphilis), Toxoplasma Gond and Cytomegalovisus were performed for patients. After examining the results of the test and excluding all risk cases, 2 to 5 ml bone marrow aspiration were prepared and were transferred to the laboratory under sterile conditions in a heparin-containing tube.