MSC harvest:
After 4 weeks of culture (approximately 28 days), MSCs were harvested
via harvest medium (including 5% PBS and FBS) and centrifuged (800xg
for 15 minutes) and then were suspended in administration buffer (PBS
and 2.5% human albumin). MSCs were then washed and counted and diluted
(2x106 cells/ml) before freezing in administration buffer.