4 Bioprocessing of rAAV Using The IC-BEVS Manufacturing Platform
Besides the molecular design of expression systems, the bioprocessing
aspect of IC-BEVS is equally essential for successful manufacturing of
AAV. In a typical IC-BEVS process, the cellular state depends on the
cell-virus interaction and infection kinetics, cellular energetics and
metabolism, and extracellular culture environment. The overall outcome
is a result of multifactorial interactions under a dynamic and
productive environment of the cell culture process. The following
section will discuss the effect of these parameters on the overall
production performances of rAAV vectors in insect cells.