a Cell density at the time of infection
b Value reported for per milliliter of cell culture
c Determined by infecting HEK293 cells and subsequent
analysis under a fluorescence microscope for GFP positive cells.
Originally reported as
transducing units (TU).
d Determined via infecting HEK293 EBNA cells with AAV
vector in the presence of a helper virus and subsequent analysis via
flow cytometry for
GFP positive cells
e Total virus capsids (Cp) analyzed via ELISA
f Value of rAAV5-hGFP using Rep52 of Serotype 1.
g Value of rAAV5-hGFP generated via AAV2 VP1 domain
swapping (VP1 2/5) and Rep52 of serotype 1
h Analyzed via infecting adherent HEK293 cells and
subsequent analysis via flow cytometry
i The peak cell density during production run
j Value reported per milliliter of AVB-Sepharose
affinity-purified eluate fraction
k Determined via infecting HEK293A cells via AAV in
the absence of helper virus and subsequent analysis using a fluorescence
microscope
l Titer value reported from the analysis of one-step
AVB-Sepharose Affinity purified sample
m Analyzed via infecting suspension adapted HEK293
cells with AAV in the presence of helper virus and subsequent analysis
by flow cytometry
n Determined via analytical ultracentrifugation
analysis of one-step AVB-Sepharose Affinity purified sample and
originally reported as enhanced
transduction units (ETU)
NA: Not available in the published report
ND: Not determined
GFP: Green Fluorescence Protein
hGFP: Humanized Green Fluorescence Protein
STB: Stirred-tank bioreactor