4 Bioprocessing of rAAV Using The IC-BEVS Manufacturing Platform
Besides the molecular design of expression systems, the bioprocessing aspect of IC-BEVS is equally essential for successful manufacturing of AAV. In a typical IC-BEVS process, the cellular state depends on the cell-virus interaction and infection kinetics, cellular energetics and metabolism, and extracellular culture environment. The overall outcome is a result of multifactorial interactions under a dynamic and productive environment of the cell culture process. The following section will discuss the effect of these parameters on the overall production performances of rAAV vectors in insect cells.