4.1 Insect Cell Lines
Various insect-derived cell lines are currently being used for the manufacturing of recombinant proteins. As previously discussed Sf9 cells are predominantly used in AAV production process. Meghrous et al. studied the AAV production in Sf9 and Hi5 cells using Three-Bac system[48]. The study suggested that both cell lines offered comparable cell-specific yields of functional AAV particles. Due to better suitability of Sf9 for baculovirus stock generation over Hi5 cells[49] and no significant advantage of Hi5 over Sf9 in overall AAV yield, Sf9 cells were selected for further process development work (Table 1)[48]. Another study involving AAV production in Sf21 using a triple BV co-infection strategy also suggested better performance of Sf9 for AAV production[50].