Update of the Lipase Engineering Database
We focus only on validated enzymes acting on the synthetic polymers PET and PUR. Detailed biochemical data of catalytically active enzymes (Tables 1, S1-S3 ), analysed sequences and structures of homologous proteins are comprised in the Plastics-Active Enzymes Database and accessible at https://www.pazy.eu. Within the PAZy infrastructure, the Lipase Engineering Database (LED, https://led.biocatnet.de) serves as the database for protein sequences and structures from different superfamilies of α/β-hydrolases and their sequence annotations, since all currently known enzyme activities towards PET or PUR were reported for α/β-hydrolases.
The updated LED contains 283,672 sequence entries and 1590 PDB entries (an increase of 3034 and 33 entries compared to the previously published LED version from June 2019, respectively). For the update of the LED, sequences that shared at least 50% similarity were assigned to the samesuperfamily (Table S5 ). Sequences that shared at least 60% similarity were assigned to a homologous family ; otherwise, they were assigned to a separate group containing all “singleton” sequences. A new superfamily was introduced for PURase homologues of PudA from Delftia acidovorans as outlined in more detail below.