Update of the Lipase Engineering Database
We focus only on validated enzymes acting on the synthetic polymers PET
and PUR. Detailed biochemical data of catalytically active enzymes
(Tables 1, S1-S3 ), analysed sequences and structures of
homologous proteins are comprised in the Plastics-Active Enzymes
Database and accessible at https://www.pazy.eu. Within the PAZy
infrastructure, the Lipase Engineering Database (LED,
https://led.biocatnet.de) serves as the database for protein sequences
and structures from different superfamilies of α/β-hydrolases and their
sequence annotations, since all currently known enzyme activities
towards PET or PUR were reported for α/β-hydrolases.
The updated LED contains 283,672 sequence entries and 1590 PDB entries
(an increase of 3034 and 33 entries compared to the previously published
LED version from June 2019, respectively). For the update of the LED,
sequences that shared at least 50% similarity were assigned to the samesuperfamily (Table S5 ). Sequences that shared at least
60% similarity were assigned to a homologous family ; otherwise,
they were assigned to a separate group containing all “singleton”
sequences. A new superfamily was introduced for PURase homologues of
PudA from Delftia acidovorans as outlined in more detail below.