Microbial host, enzyme,gene d
Ref.
Genbank, Uniprot IDa PDB structure
WT Enzymes and variants tested
Activity on PET foil,powder, bottleb
Proteobacteria-affiliated PET active enzymes
Ideonella sakaiensis 201-F6, ISPETase, ISF6_4831
53
A0A0K8P6T7
WT
54
5XFZ 5XFY 5XG0 5XH2 5XH3 R103G/S131A, S185H, C174S, C210S, W156A, S131A, I179A, W130A, M132A, Y58A, T59A R132G/S160A
55
6EQE 6EQD 6EQG 6EQF 6EQH
W185A, S238F/W159H
+, crystallinity changes observed
39,56
5XJH 5YNS
R280A, C203A/C239A
+, only relative data using 200 nM of enzyme
57
6IJ4 6IJ3 6IJ6 6IJ5
S160A, D206A, H237A, Y87A, M161A, W185A, I208A, W159A, S238A, N241A, R280A, W159H, S238F
14-fold increased activity of S121E/D186H/R280A over WT; 83 µM TA and 37µM MHET released after 1-10 days
58 6ILW N241F, W159F, S93M n.d.
38 6ANE C176A, C212A, C176A-C212A n.d.
PET4, Marinobacter nanhaiticus D15-8W, Gene: J057_15340 50 N6VY44_9ALTE WT enzyme +
PET5, Oleispira antarctica RB-8 LipA
50
R4YKL9_OLEAN
WT enzyme
+
PET6, Vibrio gazogenes Gene: BSQ33_03270
50
A0A1Z2SIQ1_VIBGA
WT enzyme
+
PET12, Polyangium brachysporum Gene: AAW51_2473
50
A0A0G3BI90_9BURK
WT
+
P.pseudoalcaligenes, DSM 50188 PpCutA
59
KU695574
n.d.
P. pelagia, DSM 25163 PpelaLip
59
KU695573
n.d.
P. aestusnigri VGXO14, B7O88_11480, PE-H
60
A0A1H6AD45
6SBN
WT, Y250S S171A, D217A, H249A, G254S, S256N,257S,Y258N,N259Q,ext.loop,Y250S,Q294A,I219Y 6 mg MHET/L from PET film (WT and Y250S) in 24h x 500nM enzyme-1 ; and 0.1 mg/ MHET from PET bottle for Y250S in 24h x 500nM enzyme-1
P. mendocina ATCC 53552 PMC 61 N20M5AZM016 2FX5 solubilized 250 µm thick films in 96 hours
Actinobacterial enzymes
LCC, leaf compost metagenome
62
G9BY57 AEV21261.1
12 mg TAeq. X h−1 x mg enzyme−1 with WT enzyme
63 4EB0
64 N197Q, N266Q, N239G, LCC-G
40
6THS 6THT S165A, ICCG-S165A F243I/D238C/S283C/ Y127G (ICCG), F243I/D238C/S283C/N246M (ICCM), F243W/D238C/ S283C/Y127G (WCCG) and F243W/D238C/S283C/N246M (WCCM),
105.6 ± 3.9 mg TAeq. X h−1 x mg enzyme−1, on commercial GF-Pet with best variant
BhrPETase from HRB29 bacterium
T. fusca DSM43793 TfH, BTA-1
65, 66, 67, 68
Q6A0I4_THEFU AJ810119
WT
+
T. fusca DSM43793 TfH, BTA-2
65, 66, 67, 68
AJ810119
+
T. fusca DSM 44342, TfH42_Cut1
69
E9LVI0_THEFU ADV92528.1
41 mmol TA/mmol enzyme in 120 hours
T. fusca, (strain xy) WSH03-11, Tfu_0882 70, 71, 72 Q47RJ7_THEFY n.d.
T. fusca, (strain xy) WSH03-11, Tfu_0883 70 Q47RJ6_THEFY n.d.
T. fusca, TfCut-2 (Cut2-kw3) 73 E5BBQ3_THEFU G62A/F209A 31 ± 0.1 nmol min-1 cm-2 on films; 12-fold better than WT; lcPET film (200 μm) 97 ± 1.8% in 30 h
74
4CG1 4CG2 4CG3
75
4CG1
G62A/I213S, G62A
42% weight loss after 50 h on film G62A/I213S, G62A 2.7-fold better than WT
T. fusca NTU22, TfAXE 76 ADM47605.1 n.d.
T. fusca NRRL B-8184, Cut1 77 JN129499.1 n.d.
T. fusca NRRL B-8184, Cut2 77 JN129500.1 n.d.
T. cellulosilytica DSM44535, Thc_Cut1 78, 69 ADV92526.1 5LUI 56 mmol TA/mmol enzyme in 120 hours
T. cellulosilytica DSM44535, Thc_Cut2
78, 69
ADV92527.1
5LUJ 5LUL 5LUK R29N/A30V, R19S/R29N/A30V
5 mmol TA/mmol enzyme in 120 hours
T. alba AHL119, Est119, est2
79
F7IX06
6AID 3WYN 3VIS
n.d.
T. curvata, DSM43183 Tcur_1278
80
D1A9G5 ACY96861.1
n.d.
T. curvata, DSM43183 Tcur0390
80
ACY95991.1
n.d.
T. halotolerans, Thh_Est
81
H6WX58
21.5 mmol TA released /mol enzyme;2 hours
T. alba, Est1 (Hydrolase 4)
82
BAI99230
n.d.
Saccharomonospora (Thermoactinomyces) viridis AHK190
83, 83, 84
W0TJ64 AB728484 4WFK 4WFI 4WFJ S226P, S226P/R228S, Q138A/D250C-E296C/Q123H/N202H, disulfide bonds at N250 and E296,
30% increase in Q138A/D250C-E296C/Q123H/N202H variant, but no kinetic data
Firmicutes
Bacillus subtilis 4P3-11, BsEstB 85 ADH43200.1 nd
Bacteroidetes
Aequorivita sp. CIP111184,PET27 29 WP_111881932 Active on foil and powder
Kaistella (Chryseobacteriu) jeonii, PET30 29 WP_039353427 7PZJ Active on foil and powder
Metagenome, probably bacterial and phylogenetically unassigned
PET2, from metagenome, affiliated no obvious affiliation, lipIAF5-2
50
C3RYL0 ACC95208.1
+
PET active enzymes from Eukaryotes
Candida antarctica lipase B, CalB
61
LIPB_PSEA2
WT
solubilized 250 µm thick films in 96 hours
86 WT +
Fusarium solani FsC
61
AAA33335.1
1OXM
WT
solubilized 250 µm thick films in 96 hours
Thermomyces (Humicola) insolens HiC
61
A0A075B5G4
4OYY, 4OYL
WT
solubilized 250 µm thick films in 96 hours
86 WT +