PURase homologues
Four protein sequences for enzymes with known activity against PUR
served as queries for BLAST (blastp, version 2.10.0+) against the NCBI
non-redundant (nr) protein database and the PDB 21.
BLAST performance was improved by multithreading with GNU/Parallel
(version 20170622-1) 22. The BLAST results were
filtered by an E-value threshold of 10-10 and a
minimal coverage of 50% to further update the LED.