Proteobacteria-affiliated PET active enzymes |
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Ideonella sakaiensis 201-F6,
ISPETase, ISF6_4831
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53
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A0A0K8P6T7
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WT
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54
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5XFZ
5XFY
5XG0
5XH2
5XH3
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R103G/S131A, S185H, C174S, C210S, W156A, S131A, I179A, W130A, M132A,
Y58A, T59A
R132G/S160A
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55
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6EQE
6EQD
6EQG
6EQF
6EQH
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W185A, S238F/W159H
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+, crystallinity changes observed
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39,56
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5XJH
5YNS
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R280A, C203A/C239A
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+, only relative data using 200 nM of enzyme
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57
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6IJ4
6IJ3
6IJ6
6IJ5
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S160A, D206A, H237A, Y87A, M161A, W185A, I208A, W159A, S238A, N241A,
R280A, W159H, S238F
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14-fold increased activity of S121E/D186H/R280A over WT; 83 µM TA and
37µM MHET released after 1-10 days
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58 |
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6ILW |
N241F, W159F,
S93M |
n.d. |
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38 |
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6ANE |
C176A, C212A,
C176A-C212A |
n.d. |
PET4, Marinobacter nanhaiticus D15-8W, Gene: J057_15340 |
50 |
N6VY44_9ALTE |
|
WT enzyme |
+ |
PET5, Oleispira antarctica RB-8
LipA
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50
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R4YKL9_OLEAN
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WT enzyme
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+
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PET6, Vibrio gazogenes
Gene: BSQ33_03270
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50
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A0A1Z2SIQ1_VIBGA
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WT enzyme
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+
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PET12, Polyangium brachysporum
Gene: AAW51_2473
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50
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A0A0G3BI90_9BURK
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WT
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+
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P.pseudoalcaligenes, DSM 50188
PpCutA
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59
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KU695574
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n.d.
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P. pelagia, DSM 25163
PpelaLip
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59
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KU695573
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n.d.
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P. aestusnigri VGXO14,
B7O88_11480,
PE-H
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60
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A0A1H6AD45
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6SBN
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WT, Y250S
S171A, D217A, H249A, G254S,
S256N,257S,Y258N,N259Q,ext.loop,Y250S,Q294A,I219Y
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6 mg MHET/L from PET film (WT and Y250S) in 24h x 500nM
enzyme-1
; and 0.1 mg/ MHET from PET bottle for Y250S in 24h x 500nM
enzyme-1
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P. mendocina ATCC 53552 PMC
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61 |
N20M5AZM016 |
2FX5 |
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solubilized 250 µm thick films in 96 hours |
Actinobacterial enzymes |
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LCC, leaf compost metagenome
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62
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G9BY57
AEV21261.1
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12 mg TAeq. X h−1 x mg
enzyme−1 with WT enzyme
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63 |
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4EB0 |
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64 |
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N197Q, N266Q, N239G, LCC-G |
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40
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6THS
6THT
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S165A, ICCG-S165A
F243I/D238C/S283C/ Y127G (ICCG), F243I/D238C/S283C/N246M (ICCM),
F243W/D238C/ S283C/Y127G (WCCG) and F243W/D238C/S283C/N246M
(WCCM),
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105.6 ± 3.9 mg TAeq. X h−1 x mg
enzyme−1, on commercial GF-Pet with best variant
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BhrPETase from HRB29 bacterium |
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T. fusca DSM43793
TfH, BTA-1
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65, 66, 67,
68
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Q6A0I4_THEFU
AJ810119
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WT
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+
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T. fusca DSM43793
TfH, BTA-2
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65, 66, 67,
68
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AJ810119
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+
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T. fusca DSM 44342,
TfH42_Cut1
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69
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E9LVI0_THEFU
ADV92528.1
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41 mmol TA/mmol enzyme in 120 hours
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T. fusca, (strain xy) WSH03-11, Tfu_0882 |
70,
71, 72
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Q47RJ7_THEFY |
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n.d. |
T. fusca, (strain xy) WSH03-11, Tfu_0883 |
70 |
Q47RJ6_THEFY |
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n.d. |
T. fusca, TfCut-2 (Cut2-kw3) |
73 |
E5BBQ3_THEFU |
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G62A/F209A |
31 ± 0.1 nmol
min-1 cm-2 on films; 12-fold better
than WT; lcPET film (200 μm) 97 ± 1.8% in 30 h |
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74
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4CG1
4CG2
4CG3
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75
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4CG1
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G62A/I213S, G62A
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42% weight loss after 50 h on film
G62A/I213S, G62A 2.7-fold better than WT
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T. fusca NTU22, TfAXE |
76 |
ADM47605.1 |
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n.d. |
T. fusca NRRL B-8184, Cut1 |
77 |
JN129499.1 |
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n.d. |
T. fusca NRRL B-8184, Cut2 |
77 |
JN129500.1 |
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n.d. |
T. cellulosilytica DSM44535, Thc_Cut1 |
78,
69
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ADV92526.1 |
5LUI |
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56 mmol
TA/mmol enzyme in 120 hours |
T. cellulosilytica DSM44535, Thc_Cut2
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78, 69
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ADV92527.1
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5LUJ
5LUL
5LUK
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R29N/A30V,
R19S/R29N/A30V
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5 mmol TA/mmol enzyme in 120 hours
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T. alba AHL119, Est119, est2
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79
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F7IX06
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6AID
3WYN
3VIS
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n.d.
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T. curvata, DSM43183
Tcur_1278
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80
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D1A9G5
ACY96861.1
|
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n.d.
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T. curvata, DSM43183
Tcur0390
|
80
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ACY95991.1
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n.d.
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T. halotolerans, Thh_Est
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81
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H6WX58
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21.5 mmol TA released /mol enzyme;2 hours
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T. alba, Est1 (Hydrolase 4)
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82
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BAI99230
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n.d.
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Saccharomonospora (Thermoactinomyces) viridis AHK190
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83, 83, 84
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W0TJ64
AB728484
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4WFK
4WFI
4WFJ
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S226P, S226P/R228S,
Q138A/D250C-E296C/Q123H/N202H, disulfide bonds at N250 and E296,
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30% increase in Q138A/D250C-E296C/Q123H/N202H variant, but no kinetic
data
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Firmicutes |
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Bacillus subtilis 4P3-11, BsEstB |
85 |
ADH43200.1 |
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nd |
Bacteroidetes |
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Aequorivita sp. CIP111184,PET27 |
29 |
WP_111881932 |
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Active on foil and powder |
Kaistella (Chryseobacteriu) jeonii, PET30
|
29 |
WP_039353427 |
7PZJ |
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Active on foil and powder |
Metagenome, probably bacterial and phylogenetically unassigned |
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PET2, from metagenome, affiliated no obvious affiliation,
lipIAF5-2
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50
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C3RYL0
ACC95208.1
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+
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PET active enzymes from Eukaryotes |
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Candida antarctica lipase B,
CalB
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61
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LIPB_PSEA2
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WT
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solubilized 250 µm thick films in 96 hours
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86 |
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WT |
+ |
Fusarium solani
FsC
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61
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AAA33335.1
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1OXM
|
WT
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solubilized 250 µm thick films in 96 hours
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Thermomyces (Humicola) insolens
HiC
|
61
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A0A075B5G4
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4OYY,
4OYL
|
WT
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solubilized 250 µm thick films in 96 hours
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86 |
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WT |
+ |