Table 1: Treatment combinations and respective sample sizes Table 1: Treatment combinations and respective sample sizes Table 1: Treatment combinations and respective sample sizes Table 1: Treatment combinations and respective sample sizes Table 1: Treatment combinations and respective sample sizes
Treatment combination DESS vs ETOH Heat treated v. untreated Cold v. Room temperature N
1 D H C 5
2 D H R 5
3 D N C 5
4 D N R 5
5 E H C 5
6 E H R 5
7 E N C 5
8 E N R 5
Notes: DESS: solution of 20% DMSO, 0.25 M EDTA and NaCl saturated. ETOH: 99.5% ethanol. Heat-treated samples were kept at 80°C for 5 min within 1 hour of sampling. Samples kept at “cold” temperatures were stored at 5°C. All samples were stored under dry dark conditions. N: number of tissue samples for a specific treatment. Notes: DESS: solution of 20% DMSO, 0.25 M EDTA and NaCl saturated. ETOH: 99.5% ethanol. Heat-treated samples were kept at 80°C for 5 min within 1 hour of sampling. Samples kept at “cold” temperatures were stored at 5°C. All samples were stored under dry dark conditions. N: number of tissue samples for a specific treatment. Notes: DESS: solution of 20% DMSO, 0.25 M EDTA and NaCl saturated. ETOH: 99.5% ethanol. Heat-treated samples were kept at 80°C for 5 min within 1 hour of sampling. Samples kept at “cold” temperatures were stored at 5°C. All samples were stored under dry dark conditions. N: number of tissue samples for a specific treatment. Notes: DESS: solution of 20% DMSO, 0.25 M EDTA and NaCl saturated. ETOH: 99.5% ethanol. Heat-treated samples were kept at 80°C for 5 min within 1 hour of sampling. Samples kept at “cold” temperatures were stored at 5°C. All samples were stored under dry dark conditions. N: number of tissue samples for a specific treatment. Notes: DESS: solution of 20% DMSO, 0.25 M EDTA and NaCl saturated. ETOH: 99.5% ethanol. Heat-treated samples were kept at 80°C for 5 min within 1 hour of sampling. Samples kept at “cold” temperatures were stored at 5°C. All samples were stored under dry dark conditions. N: number of tissue samples for a specific treatment.