Figure 4. Genome-wide genetic differentiation (FST), regional variation in absolute divergence (DXY), Tajima’s D (TD) and nucleotide diversity (θπ) in eastern and western monarchs. These summary statistics were calculated in non-overlapping windows of 10kb across the genome. The alternating gray blocks represent different chromosomes in the genome. FST andDXY are between-group comparisons, and a single yellow line is shown for these measures. In contrast, Tajima’s D (Td ) and nucleotide diversity (θπ ) are group-specific measures, and eastern and western monarchs are indicated with black and red respectively; due to genome-wide overlap, the black (eastern) data are mostly hidden behind the red (western) data.