Sequence analysis of the BLK
We have retrieved the data of Human BLK from uniport
(https://www.uniprot.org/uniprot/P51451) and analysed the sequence of
BLK using Geneious Prime (https://www.geneious.com/prime/) for the basic
quality assessment. Our sequence is having charge of 2.21 at pH 7 and
isoelectric point is 7.61. Frequency of Acidic residues is 66 which is
13.1% of the sequence, frequency of basic residues is 76 which is 15%,
Polarly uncharged and charged are 121 and 142 which are 24% and 28.1%
respectively. Hydrophobic residues are 252 in number which is making
49.9% of the total sequence.ProtParam
(Gasteiger et al., 2005), EMBOSS plugin
(Rice, Longden, & Bleasby, 2000)and have
arranged the data using excel and plotted using tableau. Computer-based
clustering analysis of kinase is picking up in significance as an
expository apparatus, it is getting conventional for the investigation
because of the accessibility of quick increment in the succession.In a
complete sequence of analysis of BLK, we have found, Lysine is having
the highest frequency of total 68 times and Arginine is second highest
of 66residues and both are negatively charged. Cysteine
(sulphur-containing amino acid) and histidine which considered to be an
inflammatory agent in immune response whichare the lowest occurring
amino acid andare eight in numbers (Fig. 2).
Fig. 2 Amino acid distribution throughout the sequence of BLK