Sequence analysis of the BLK
We have retrieved the data of Human BLK from uniport (https://www.uniprot.org/uniprot/P51451) and analysed the sequence of BLK using Geneious Prime (https://www.geneious.com/prime/) for the basic quality assessment. Our sequence is having charge of 2.21 at pH 7 and isoelectric point is 7.61. Frequency of Acidic residues is 66 which is 13.1% of the sequence, frequency of basic residues is 76 which is 15%, Polarly uncharged and charged are 121 and 142 which are 24% and 28.1% respectively. Hydrophobic residues are 252 in number which is making 49.9% of the total sequence.ProtParam (Gasteiger et al., 2005), EMBOSS plugin (Rice, Longden, & Bleasby, 2000)and have arranged the data using excel and plotted using tableau. Computer-based clustering analysis of kinase is picking up in significance as an expository apparatus, it is getting conventional for the investigation because of the accessibility of quick increment in the succession.In a complete sequence of analysis of BLK, we have found, Lysine is having the highest frequency of total 68 times and Arginine is second highest of 66residues and both are negatively charged. Cysteine (sulphur-containing amino acid) and histidine which considered to be an inflammatory agent in immune response whichare the lowest occurring amino acid andare eight in numbers (Fig. 2).
Fig. 2 Amino acid distribution throughout the sequence of BLK