Secondary structure prediction
Structural analysis is one of the major fields and it helped in the
identification of many ligand molecules for the proper binding with the
receptor (Sliwoski et al., 2014;
Kaul et al., 2019). We have got the 23
alpha-helix which considered energetically favourable for
hydrogen-bonding, 29 beta-strand which are generically more rigid than
other components, 36 coils and 36 turns which considered to contain
unusual conformational abilities. We have plotted sequence and secondary
structure parallelly using Geneious prime (Geneious 2020.0.5
(https://www.geneious.com)) (Fig. 3). We have got the length
ranges from 2-27 and the maximum length of the alpha helix is from
357-383. Beta strand is showing the length complexity from 1-14. While
the turn and coil are showing the length ranges from 1-7 residues (Fig.
4) Aloha helix are made more hydrogen bonds while the beta-strand are
giving high stability to the structure. From Ramachandran plot, we have
found there is Cis proline is present which is 2/24 (A306 GLU-A307 PRO),
(A504 GLN-A505 PRO) and Ramachandran outliers which is 1.13% A501
TYR, A500 GLN, A496 ALA, A386 ASP, A142 ILE.
Fig. 3 Count of Secondary structure components
Fig. 4 Secondary structure of Human BLK