Materials and Methods
We have done various deep analysis on human BLK sequence, homology modelling is the development of an atomic version of a target protein primarily based totally at the goal’s amino acid series and the templates; experimentally determined systems of homologous proteins. In recent years structural biology shifted towards structure-based drug discovery. We have modelled the structure using the techniques of homology modelling (Jacobson et al., 2004; Waterhouse et al., 2018). The structure was pre-processed using the protein preparation wizard and have filled the gap using Prime, the further structure was minimized in terms of its energy using the same wizard for the next step of the analysis. The active site is the region of an enzyme in which substrate molecules bind and go through a chemical reaction. The lively site consists of residues that shape brief bonds with the binding site and residues that catalyse a response of the catalytic site, prediction of the active site of the BLKhave bee done using SiteMap (Singh et al., 2011; Wass et al., 2010) with the specific parameters of more restrictive and standard grid of crops site of 4 maps for the further analysis and have got total of 5 active site. We have also extracted the amino acid distribution throughout the sequence using the ProtParam and got the CSV format data after that we have performed various analysis in terms of statistical analysis and have plotted the data for the quick understanding of the BLK. ProtParam lets in the computation of diverse bodily and chemical parameters such as molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, estimated half-life, instability index, aliphatic index and grand average of hydropathicity for a given protein saved in Swiss-Prot or TrEMBL or for a user-entered protein sequence(Gasteiger et al., 2005). Secondary structure of the protein has been predicted using emboss (Garnier et al., 1978). GeneCards server was used for the categorisation of mRNA expression level in different human tissues. Antigenic regions on protein defined a lot about inhibitors and the future research scope on that particular protein. In our studies Emboss was used for the extraction of the antigenic region and Geneious prime was used for the plotting of the data. Additionally, we have also used InterProScan for the characterisation of the domains on the surface of BLK for better functional understanding. We have collected BLK from thousands of species and have mined the best, based on the characterisation and have taken the only largest size of BLK from every species and got a total of 324 species. Further data have been aligned using MAFFT v7.450 Alignment (Rozewicki et al., 2019). We have used FFT-NS-1 and 200PAM/k=2 as main principle parameters and have got comparative annotations of every aspect of the various BLK. Geneious Tree has performed for the plotting of the tree with automatic direction determiner. Further, Itol server (Letunic & Bork, 2019) used for editing of a phylogenetic tree for better understanding with its annotation characterization and bootstrapping.