Secondary structure prediction
Structural analysis is one of the major fields and it helped in the identification of many ligand molecules for the proper binding with the receptor (Sliwoski et al., 2014; Kaul et al., 2019). We have got the 23 alpha-helix which considered energetically favourable for hydrogen-bonding, 29 beta-strand which are generically more rigid than other components, 36 coils and 36 turns which considered to contain unusual conformational abilities. We have plotted sequence and secondary structure parallelly using Geneious prime (Geneious 2020.0.5 (https://www.geneious.com)) (Fig. 3). We have got the length ranges from 2-27 and the maximum length of the alpha helix is from 357-383. Beta strand is showing the length complexity from 1-14. While the turn and coil are showing the length ranges from 1-7 residues (Fig. 4) Aloha helix are made more hydrogen bonds while the beta-strand are giving high stability to the structure. From Ramachandran plot, we have found there is Cis proline is present which is 2/24 (A306 GLU-A307 PRO), (A504 GLN-A505 PRO) and Ramachandran outliers which is 1.13% A501 TYR, A500 GLN, A496 ALA, A386 ASP, A142 ILE.
Fig. 3 Count of Secondary structure components
Fig. 4 Secondary structure of Human BLK