Analysis of Isoelectric point and charges of the residues
We have analysed isoelectric point and charges of every residue on blk using the Geneious prime. This plot can clearly define the Hydrophobicity of the residues along with its isoelectric point, both play an important role in drug designing. We have also categorised the Transmembrane permeability and plotted its affinity at outside, inside and on the plasma membrane of the cell. An amino acid charge is basically defining the charge per residues which can be added for the cumulative analysis of the receptor. The cumulative of hydrophobicity at each residue could reveal a lot about the drug design for BLK to upregulate and downregulate in the human body. This graph is depicting the charge, TM along with the secondary structure for the better understanding of the system. For the detailed analysis of the domains of the Human BLK, we have plotted all the annotations along with the domain region on the sequence (Fig. 11).
Fig. 11 Graphical representation of annotations of the BLK