Primary striatal neuron cultures
Primary cultures of embryonic (E) day 14 rat ganglionic eminence
(hereafter referred to as striatum) (from the Biological Service Unit,
University College Cork) were dissected as previously described
(Schmidt et al. , 2012). All
scientific procedures were performed under a license in accordance with
the European Communities Council Directive (86/609/EEC) and approval by
local Animal Experimentation Ethics Committee. After dissection, the
tissue was dissociated and neurons were plated in poly-D-lysine coated
24-well plates (Sigma) in DMEM:F12 media (Sigma) supplemented with 1%
penicillin/streptomycin (Sigma), 1% L-glutamine (Sigma), 2% B27
(Invitrogen) and 1% FBS. Cells were maintained in culture for 7DIV.