3RAD Data
For the 157 specimens sampled throughout the distribution of theA. unicolor species complex, 234,116,852 raw reads were obtained ranging from 365,011 to 4,698,473 and averaging 1,491,190 reads per sample (Table 1). Table 1 outlines the number of loci and SNPs generated in each dataset with different min_sample_locus parameter values. Additional clustering thresholds (0.85, 0.88, 0.90) were tested as well, but there were minimal differences in the number of retained loci/SNPs across these thresholds; therefore, we chose the threshold value of 0.85 in ipyrad for generating our data matrices. When min_sample_locus values are lower for both the full dataset (All_samples) and the subset with A. unicolor B samples only (A.unicolorB_only), more loci and SNPs are retained compared to higher min_sample_locus values, which have a lower yield of loci and SNPs.