Genetic Exchangeability - Clustering Analyses
Three different genetic clustering analyses were used to evaluate the
potential for gene flow (see Table 2 for summary). The STRUCTURE
analysis with All_M20 resulted in an optimal K value of eight (Figure
3a), with the two monophyletic lineages A. microunicolor andA. unicolor A clustering separately; however, A. unicolor B
formed six clusters that did not correspond to monophyletic groups.
STRUCTURE analyses with UniB-only matrices produce two to three clusters
with all but one data set recovering non-monophyletic clusters (Figure
4). UniB_M30 does recover the three monophyletic lineages withinA. unicolor B, but some individuals have mixed cluster assignments
(Figure 4). In contrast, both PCA and VAE clustering analyses (Figure
3b, 3c, & 3d) for All-M20 indicate three distinct clusters consistent
with monophyletic lineages: A. microunicolor , A.
unicolor A, and A. unicolor B. The VAE analysis with UniB_M30
shows some separation between A. unicolor B lineages; however,
there is still some overlap between them when considering standard
deviation around samples (Figure 4). The lineages within A.
unicolor B were not recovered as discrete clusters in any of the three
clustering analyses.