Determination of quercitrin contents in bamboo
B. fargesii and F. qinlingensis samples were collected
from the Qinling Mountains, and B. faberi and F. denudatasamples were collected from the Qionglai and Minshan Mountains.
Perennial bamboo leaves were freeze-dried and crushed. The purity of the
quercitrin (Sigma-Aldrich, USA) used as the standard sample was greater
than 98%. A 50 mg sample was added to 1 ml methanol, and extraction was
performed at 65°C for 4 h. The LC-ESI-MS analysis method was used in
this experiment. A ultra high-performance liquid chromatography system
was used in the chromatographic system. According to the properties of
flavonoids, the reaction conditions were as described previously (Wu et
al., 2010). The external standard method was used for quantitative
analysis with three repetitions of each sample, and the fitting curve
was prepared by using different concentrations of standard samples. The
dose–response curves were corrected for background fluorescence and
normalized to the maximal response observed.