Analysis of transcriptional activity
The pDEST-GBKT7-SmGRAS5 and negative control plasmids were transformed into the yeast AH109. The positive control plasmid waspGBKT7-53+pGADT7-T . First, screened the transformed AH109 on synthetic dropout (SD) medium lacking tryptophan (SD/-Trp) and then on SD medium without tryptophan, histidine and adenine (SD/-Trp/-His/-Ade).