Dual-luciferase assay
The 649-bp promoter of SmKSL1 was cloned and inserted intopGREEN . The vector pCAMBIA1304-SmGRAS5 was transferred into Agrobacterium strain GV3101. pCAMBIA1304 empty vector was used as a negative control and The 35S promoter-drivenRenilla luciferase as an internal control. The two GV3101 strains were co-infiltrated into tobacco leaves. Infiltrated leaves were incubated in darkness for 8h and then in light for 40h. Three biological replicates of each sample were assayed using the Dual-Luciferase Reporter Assay System (Promega, USA).