Y1H verification
The ORF of SmGRAS5 was inserted into the pGADT7 vector.
The 649-bp SmKSL1 and 826-bp SmCPS1 promoter sequences
were cloned into the pBait-AbAi vector. The primers for vectors
(pBait-AbAi-SmKSL1-649 and pBait-AbAi-SmCPS1-826 ) are
listed in Table S2. Aureobasidin A suppressed the basal expression of
the Y1H-pAbAi-SmKSL1-649 (PYK649 ) andY1H-pAbAi-SmCPS1-649 (PYC826) yeast strain (Bai et
al. , 2018). pGADT7-SmGRAS5 was verified by interactions withPYK649 and PYC826 yeast strains, which recombined theSmKSL1 and SmCPS1 promoter in SD/-Leu/AbA.