Protein extraction and western blot
The ORF of SmGRAS5 was cloned into the pMAL-c2X vector.
The primers for vectors are listed in Table S2. The plasmids were
transformed and proteins were expressed inEscherichia
coli (Rosetta strain). Protein induction and purification were
performed as previously described (Bai et al. , 2018). The MBP
(malE) and SmGRAS5-MBP purified proteins were conducted and SDS-PAGE
analyses showed major bands with approximate 80.7 kDa molecular mass
(Fig. S7). The western blot was performed as previously described (Ruet al. , 2017).