Subcellular localization and transcriptional activity analysis
of SmGRAS5
To determine the subcellular localization of SmGRAS5, SmGRAS5 protein
was fused with a green fluorescent protein (GFP) label. The protoplasts
of tobacco leaves and onion epidermal cells were used to analyze the
SmGRA5-GFP signal (Fig. 2). The results showed that the GFP fluorescence
of SmGRAS5 was enriched in the nucleus, indicating that SmGRAS5 might
function as a TF.
To further identify the characteristics of SmGRAS5, the transcriptional
activity of SmGRAS5 was analyzed. The results showed that the yeast withSmGRAS5-pGBKT7 and control constructs were able to grow on the
SD/-Trp medium. However, the yeast with SmGRAS5-pGBKT7 and
negative control constructs could not survive on the SD/-Trp-His-Ade
medium (Fig. S2). The results demonstrated that SmGRAS5 had no
transcriptional activity.