Confirmation of DEGs by qRT-PCR
To validate the RNA-Seq data, six
genes were randomly selected from the peach fruit DEGs for qRT-PCR
analysis using PpeIF-1A as the internal control gene. The ratio of gene
expression levels between CT and HT at
each
time point were calculated for both the RNA-Seq and qRT-PCR experiments.
As shown in Supplementary
Figure S11 , the fold-changes in base-2 logarithm were similar between
the two technologies, which confirmed the reliability of our
transcriptome measurement.