FIGURE LEGENDS
Figure 1.Nakazawaea Neighbor-Joining Phylogenetic tree.Phylogeny was built based on the combined sequences of the SSU rRNA
gene, the ITS region (including 5.8S rRNA) and the D1/D2 domains of the
LSU rRNA genes of the genus Nakazawaea . Bootstrap values are
indicated on the nodes as percentages of 1,000 replicates. The alignment
included 1,895 bp and was performed with MUSCLE. Pachysolen
tannophiluswas was used as an outgroup. The distance metric is the
number of substitutions. Bar, 20 substitutions per site.
Figure 2. Differential interference contrast micrograph of
budding cells of Nakazawaea atacamensis sp. nov. Micrograph of
budding cells grown in YPD broth after 3 days at 25°C. Bars: 5
μm. This image was obtained using differential interference contrast
(DIC) microscopy.
Figure 3. N. atacamensis genome analyses. The x-axis
depicts the number of genes on each pathway identified in the N.
atacamensis genome A. KEGG distribution based on gene prediction using
KofamKOALA. B. KEGG enrichment analysis of the carbohydrate metabolism
pathways. C. Maximum-likelihood phylogenetic tree using the 15 currently
available genomes in the Nakazawaea genus. Pachysolen
tannophilus was used as an outgroup.
Figure 4. Fermentation phenotypes in N. atacamensis. A.
Growth rate under glucose, and fructose and sucrose 2% w/v, and glucose
20% w/vas carbon sources. B. Growth rates under medium supplemented
with glucose 2% w/v and ethanol at 6 and 8% v/v. Fermentation results
in Synthetic Wine Must (SWM) with a YAN concentration of 300 mg/mL. C.
CO2 loss, D. Sugar consumption, E. Ethanol production and F. Glycerol
production. EC1118 (grey dots) and N. atacamensis (orange dots).