2.9 Animals
The male Swiss albino Wistar rats (2-3 month; 170-200 g) were obtained from Central Animal House Facility. The rats were kept in the single polyethylene cage with standard experimental condition (25±2°C; relative humidity 75±5 with 12h/12h dark and light cycle). The rats received the pellet feed and water ad libitum . All the experimental study was performed according to the Institutional guidelines.