2.7 α-amylase activity
A modified method of Lordon et al. was used for the determination of
α-amylase enzymes activity (Lordan et al., 2013). Briefly, (acarbose andPA ) was added in the sodium phosphate and incubated at room
temperature for 5 min further 1 ml of salicylic acid in both samples
were added and boiled for 5 min. The reaction mixture was cool in an ice
bath and deionized water was added to calculate the absorbance at 540
nm. The percentage inhibition was estimated as per above formula.