2.12 Biochemical analysis
The BGL was determined by glucoseoxidase–peroxidase (GOD-POD) method by blood glucose level kits. The plasma insulin level was estimated by standard kits. The biological parameters such as glucose-6-phosphatase, hexokinase and fructose-16-bisphosphatase were determined as per the reported method of Kumar et al., Ahmed et al., Hepatic parameters such as Serum glutamic oxalo acetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT) and Alkaline Phosphatase (ALP) were estimated by diagnostic kits (Span diagnostic). Lipid profiles such as high-density lipoprotein (HDL), triglyceride (TG), total cholesterol (TC), were estimated by the diagnostic kits (Span diagnostic kits) and very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) were estimated via using the following formula.
The coronary risk and atherogenic index were estimated via using the following formula
\begin{equation} \text{At}h\text{erogenic}\ \text{index}=\frac{\text{Low}\ \text{density}\ \text{lipoprotein}\ ch\text{olesterol}}{\ h\text{ig}h-\text{density}\ \text{lipoprotein}\ ch\text{olesterol}\ }\nonumber \\ \end{equation}\begin{equation} \text{Coronary}\ \text{risk}\ \text{index}=\frac{\text{Total}\ ch\text{olesterol}}{\ h\text{ig}h-\text{density}\ \text{lipoprotein}\ ch\text{olesterol}\ }\nonumber \\ \end{equation}
HOMA IR and HOMA-β were estimated via using the previously reported protocol of Ahmed et al., with slight modification (Ahmed et al., 2014a, 2015).