2.3 Extraction procedure
The primary aim to scrutinize the appropriate extraction system for plant material and find out the possible system having maximum antioxidant activity. For the extraction system, solvent nature (methanol), solvent concentration (50-100%), extraction temperature (0.5-4h) and sample to the solvent ratio (1/25-1/100) were estimated, respectively and the result was used for optimise the antioxidant extraction with using RSM methodology. The seed coats were removed and shade dry and were further crushed to form the coarse powder. This coarse powder was used for the extraction by methanol. The extracted
crude extract was dry under the vacuum to form a viscous mass.