2.5 Insilico study
Insilico docking studies were performed for understanding binding character and mode of rutin at the molecular level. Maestro (Schrodinger software suite, version 9.4) was used for docking stimulation of ruin. The ligprep application was further employed d for preparation of ligand 3D format via using build panel. Protein was obtained from protein data bank (PDB ID: 5EQG, 2G63) this study n was prepared via eliminating the hydrogen, solvent, and minimization of prepared legend. Validation was made by the standard drug (glibenclamide) to re-docked at the protein catalytic site.