Estimation of Catalase activity
Catalase activity was measured according to Aebi et al. (1984). The
composition of enzyme assay mixture consisted of 100 µL enzyme extract,
100 mM phosphate buffer (pH 7.0) and 30 mM
H2O2 in a total volume of 1.5 ml. The
H2O2 was added in the last and
absorbance was measured by UV/VIS spectrophotometer at 240 nm. The
disappearance of substrate (H2O2) by
enzymatic breakdown was monitored by decrease in absorbance till 3
minutes.